TFome Information for ZmMYB40
| TFome Name: | ZmMYB40 |
| Species: | Maize |
| TF Family: | MYB |
| Genbank ID of Template cDNA: | EC905796 |
| Corresponding TF Gene: | AAO48737 |
| Vector: | pENTR/D |
| Insert: | PCR amplified CDS from template |
| 5' Primer Name: | C21 UP | 3' Primer Name: | C21 DN |
| 5' Primer Sequence: | CAC CAT GGG GAG GGC GCC | 3' Primer Sequence: | GAGATTGTCCAGGAAGAAgag |
| 5' Primer Tm (º C): | 65 | 3' Primer Tm (º C): | 52 |
| PCR Condition: | Step 1, 98°C 30 sec melt, 1 cycle; Step 2, 98°C 10 sec melt, 52°C 30 sec anneal, 72°C 45 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; Step 4, 4°C hold. 1ul Phusion (New England Biolabs), HF buffer (NEB), MgCl2 1.5 mM final concentration, template 40pg/ul final concentration. | ||
| Nucleotide Sequence: | atggggagggcgccgtgctgcgagaaggtggggctgaagaaggggaggtggaccaaggaggaggacgaggtcctggcgag gtacatcaaggagcacggggaaggatcgtggaggtcactgcccaagaatgccgggctgctgcggtgcgggaagagctgca ggctgcggtggatcaactacctgcgggcgggtctcaagagggggaacatctcggaggaggaggaggacatgatcatcaag ctccacgccacgcttggcaacaggtggtccctgatcgccggtcacttgcccggtcgaacagacaacgagatcaaaaacta ctggaactcgcacctgagcaggcgggcggccgacttccgcgacggcgtcgtcgtcgacatcgacctcagcaagctgcccg gcggcgggaaacggcgcggcggccgggccagccggggcgccgtcgtggccgcggccaaggagaagaaggccaaggagaag gacgacaggggcaatagcaaggtcgcagaagcggagcagcagctcagggacacggaggacgacgacggcggcagcgtctc cacgccgaggcctcagtctgatgactgcggcaccgcccagagcgaagaggagcaagcgcaggccagcgccagcggcctga catccgatgggcatgggcccgaggaggaggaggaggaggacccgctggctctgagcgaggagatggtgagtgcgcttctg gccccggaaagcccaaagctggaggtgggccccgatggctcgtgcatggacagctacagtggccctccgtcaggggaaag cggctgtgggtccagtgggccttctggcgacgtggcccaggacctggacctagacgacgacaaggccatcatggactggg acttgatggggctggacatctcgaccgccggtgacatgtgggaccagctggtgtgggactacgacgaaacgttggtcacg gaaccggaaggaggggaggaagggcaccagcagcaggacgatgtcatgtcagacctcttcttcctggacaatctc | ||
| TFome clones do not contain stop codon at 3'end. | |||
| Translation: | mgrapccekvglkkgrwtkeedevlaryikehgegswrslpknagllrcgkscrlrwinylraglkrgniseeeedmiik lhatlgnrwsliaghlpgrtdneiknywnshlsrraadfrdgvvvdidlsklpgggkrrggrasrgavvaaakekkakek ddrgnskvaeaeqqlrdtedddggsvstprpqsddcgtaqseeeqaqasasgltsdghgpeeeeeedplalseemvsall apespklevgpdgscmdsysgppsgesgcgssgpsgdvaqdldldddkaimdwdlmgldistagdmwdqlvwdydetlvt epeggeeghqqqddvmsdlffldnl | ||
| Request Information: | Dr. John Gray Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606 jgray5@utnet.utoledo.edu | ||